It is now well established that this abnormal inflammatory response requires interplay between host genetic and intestinal microbiota. Several lines of evidence support the notion that CD results from an excessive immune response to gut commensal or pathogenic organisms. We are the first to report that the ileal mucosa of CD patients is abnormally colonized by adherent-invasive E. coli (AIEC).

We aim to analyze how these bacteria can be involved in the onset or the recurrence of intestinal inflammation in genetically predisposed patients. In this context, our research project will be focused on intestinal inflammation-associated E. coli strains according to three major themes: (i) analysis of intestinal microbiota associated to inflammatory disorders; (ii) better characterization of the pathogens; and (iii) study of host-bacteria cross-talk at the intestinal mucosal site.
  • Analysis of intestinal microbiota associated to inflammatory disorders
  • Molecular characterization and comparison of E. coli strains associated with intestinal inflammation
    • transcriptomic profiles of AIEC strains
    • proteomic profiles of AIEC strains
    • role of bacterial protein glycosylation in the virulence of AIEC bacteria.
  • Bacteria interaction at the inflamed intestinal mucosa in a susceptible host :
    • Influence of environmental factors
    • Interaction between Peyer's patches and E. coli
    • Innate immunity deficiency and/or modulation by E. coli in the context of inflammation
    • The role of exosomes in cell-to-cell communication and in host response to AIEC infection
    • Adaptive immunity modulation by E. coli in the context of inflammation.